Novel drugs and indications discovered with MetaMouse® and MetaMouse® GFP/RFP models
Organ-specific models-MetaMouse
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Molecular Imaging of Chemotherapy
Customized service for all your in vivo cancer drug screening, discovery, evaluation and development needs.
Develop New Effective Drugs for Metastasis and Angiogenesis

  • Green/Red fluorescent protein (GFP, RFP) tumor expression for quantitative functional imaging.
  • In vivo drug discovery and evaluation for tumor growth, metastasis, angiogenesis.
  • Whole-body functional imaging of drug efficacy on tumor growth, metastasis, angiogenesis, gene expression.
  • Real-time in vivo visualization of tumor growth, metastasis, angiogenesis, gene expression.
  • More than 100 GFP/RFP-expressing tumor models available for MetaMouse®/AngioMouse®.
  • Orthotopic metastatic MetaMouse®/AngioMouse® models.
  • All types of agents can be evaluated: small molecules, proteins, genes.
  • In vivo high-throughput drug screening and evaluation.
  • Customized contract research for all types of drug discovery and evaluation.

In The News: The New York Times about MetaMouse® technique

 

View whole metamouse PPT presentations

 

Uses for multicolored imaging in vivo  

Trafficking of tumor cells (click onto image to view video) 

Comparison of GFP and Luciferase Imaging*
Method Minimum cells imageable in vitro Minimum cells imageable in vivo Need for substrate? Need for anesthesia? Methodof visualization Multicolor imaging
GFP 1 1 No No Direct Imaging Yes
Luciferase 300 3000 Yes Yes Photon counting (pseudo-color) No
* THE LANCET Oncology 3, p546-556, 2002


Recent MetaMouse®/Oncobrite®/AngioMouse®/GeneBrite® references:
1. Yang, M., et al. Whole-body optical imaging of green fluorescent protein-expressing tumors and metastases. Proc. Natl. Acad. Sci. USA 97, 1206-1211, 2000.
2. Yang M., et al. Visualizing gene expression by whole-body fluorescence imaging. Proc. Natl. Acad. Sci. USA 97, 12278-12282, 2000.
3. Yang M., et al. Whole-body and intravital optical imaging of angiogenesis in orthotopically implanted tumors. Proc. Natl. Acad. Sci. USA 98, 2616-2621, 2001.
4. Hoffman, R.M. Orthotopic metastatic mouse models for anticancer drug discovery and evaluation: a bridge to the clinic. Investig. New Drugs, 17, 343-359, 1999
5. McCann, J. Jellyfish protein gives new glow to tumor imaging. Journal of the National Cancer Institute 93, 976-977, 2001.
6. Yang, M., Baranov, E., Wang, J-W., Jiang, P., Wang, X., Sun, F-X., Bouvet, M., Moossa, A.R., Penman, S., and Hoffman, R.M. Direct external imaging of nascent cancer, tumor progression, angiogenesis, and metastasis on internal organs in the fluorescent orthotopic model. Proc. Natl. Acad. Sci. USA 99, 3824-3829, 2002.
7. Bouvet, M., Wang, J-W., Nardin, S.R., Nassirpour, R., Yang, M., Baranov, E., Jiang, P., Moossa, A.R., and Hoffman, R.M. Real-time optical imaging of primary tumor growth and multiple metastatic events in a pancreatic cancer orthotopic model. Cancer Research 62, 1534-1540, 2002.
8. Schmitt, C.A., Fridman, J.S., Yang, M., Baranov, E., Hoffman, R.M. and Lowe, S.W. Dissecting p53 tumor suppressor functions in vivo. Cancer Cell 1, 289-298, 2002
9. Schmitt, C.A., Yang, M., Fridman, J.S., Baranov, E., Hoffman, R.M., and Lowe, S.W. Senescence program controlled by p53 and p16INK4a contributes to the outcome of cancer therapy. Cell 109, 335–346, 2002
10. Hoffman, R.M. Green fluorescent protein imaging of tumor growth, metastasis, and angiogenesis in mouse models. Lancet Oncology 3, 546-556, 2002

11. Glinskii, A.B., Smith, B.A., Jiang, P., Li, X-M., Yang, M., Hoffman, R.M., Glinsky, G.V. Viable circulating metastatic cells produced in orthotopic but not ectopic prostate cancer models. Cancer Res. 63, 4239-4243, 2003.

12. Yang, M., Li, L., Jiang, P., Moossa, A.R., Penman, S., and Hoffman, R.M.  Dual-color fluorescence imaging distinguishes tumor cells from induced host angiogenic vessels and stromal cells.  Proc. Natl. Acad. Sci. USA 100, 14259-14262, 2003.

13. Yamamoto, N., Jiang, P., Yang, M., Xu, M., Yamauchi, K., Tsuchiya, H., Tomita, K., Wahl, G.M., Moossa, A.R., and Hoffman, R.M. Cellular dynamics visualized in live cells in vitro and in vivo by differential dual-color nuclear-cytoplasmic fluorescent-protein expression. Cancer Research 64, 4251-4256, 2004.

14. Yang, M., Reynoso, J., Jiang, P., Li, L., Moossa, A.R., and Hoffman, R.M.  Transgenic nude mouse with ubiquitous green fluorescent protein _expression as a host for human tumors.  Cancer Research 64, 8651-8656, 2004.

15. Amoh, Y., Yang, M., Li, L., Reynoso, J., Bouvet, M., Moossa, A.R., Katsuoka, K., and Hoffman, R.M.  Nestin-linked green fluorescent protein transgenic nude mouse for imaging human tumor angiogenesis.  Cancer Res. 65, 5352-5357, 2005.

16. Hoffman, R.M. and Yang, M. Dual-color whole-body imaging in mice. Nature Biotechnology 23, 790-791, 2005.

17. Yang, M., Luiken, G., Baranov, E., and Hoffman, R.M. Facile whole-body imaging of internal fluorescent tumors in mice with an LED flashlight. Bio Techniques 39, 170-172, 2005.

18. Hoffman, R.M.  The multiple uses of fluorescent proteins to visualize cancer in vivo. Nature Reviews Cancer 5, 796-806, 2005.

19. Yamauchi, K., Yang, M., Jiang, P., Xu, M., Yamamoto, N., Tsuchiya, H., Tomita, K., Moosa, A.R.,  Bouvet, M., and Hoffman, R.M. Development of real-time subcellular dynamic multicolor imaging of cancer cell trafficking in live mice with a variable-magnification whole-mouse imaging system. Cancer Res. 66, 4208-4214, 2006.

20. Hoffman, R.M., and Yang, M.  Subcellular imaging in the live mouse.  Nature Protocols 1, 775-782, 2006.

21. Hoffman, R.M., and Yang, M.  Color-coded fluorescence imaging of tumor-host interactions.  Nature Protocols 1, 928-935, 2006.

22. Hoffman, R.M., and Yang, M.  Whole-body imaging with fluorescent proteins.  Nature Protocols 1, 1429-1438, 2006.

23. Bouvet, M., Tsuji, K., Yang, M., Jiang, P., Moossa, A.R., and Hoffman, R.M.  In vivo color-coded imaging of the interaction of colon cancer cells and splenocytes in the formation of liver metastases.  Cancer Research 66, 11293-11297, 2006

24. Hayashi, K., Yamauchi, K., Yamamoto, N., Tsuchiya, H., Tomita, K., Amoh, Y., Hoffman, R.M., and Bouvet, M.  Dual-color imaging of angiogenesis and its inhibition in bone and soft tissue sarcoma.  J. Surg. Res. 140, 165-170, 2007.

MetaMouse US Patents 5,491,284, 5,569,812, Japanese Patents 2,664,261, European Patent 437,488,

GFP US Patents 6,232,523, 6,235,967, 6,235,968, 6,251,384, 6,649,159, 6,759,038, 6,905,831, Japanese Patents 3,709,343 and 4,021,197, European Patents 0979298,  1156833,  1294906, Australian Patents 749,338 and 2001249297, 2003272221, Chinese Patents  03823937X, German  patent 60137501708, Korean Patent  10-868200, Canadian Patents 2,358,439 and 2289283.

MetaMouse®/AngioMouse® whole-body imaging with GFP/RFP!
Whole-body imaging of tumor angiogenesis in AngioMouse® Whole-body imaging of single glioma cells in the brain in MetaMouse®/OncoBrite®
Whole-body imaging of liver metastases in MetaMouse®/OncoBrite® Skeletal metastases in MetaMouse®/OncoBrite®
Whole-body dual color imaging of GFP and RFP mammary tumors in MetaMouse®/OncoBrite® Whole-body imaging of gene expression in the liver in GeneBrite® model
Whole-body real time imaging of metastatic growth and drug response
Drug response on human metastatic tumors growing orthotopically in mice
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