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Tumor Type CELL LINE

WHOLE-BODY IMAGING OF ORTHOTOPIC PC-3-GFP IN NUDE MICE

Bladder, human

HTB-9

UC-14

KU-7-GFP human bladder carcinoma growing on nude mouse bladder

Breast cancer, human

MDA-MB-435

MDA-MB-231

MDA-MB-468

MCF-7

MX-1

MX-1-GFP and –RFP breast carcinoma growing on mammary fat pads of SCID mouse. Whole-body image.

Primary breast tumor MDA-MB-435 and lymph node metastases expressing GFP or RFP

CNS, human

SNB-19

324

U87-MG

Colon cancer, human

HCT-116

COLO-205

SW-620

Ls-180

HCT-15

KM-12

WiDr

HT-29

CaCo-2

RKO

AC3488-GFP-tumor growing on colon and metastasized to liver and lymph nodes of nude mouse.Whole-body image.. Primary tumor - red arrow, liver metastases - white arrow, lymph node - yellow arrow.

Fibrosarcoma, human

HT-1080

Hepatoma, human

SMMC-7721

Larynx, human

Hep-2

Primary tumor growth of Hep-2

Lung cancer, human

H-460

HOP-62

EKVX

Anip-973

A549

Lung cancer, mouse

Lewis Lung

H-460-GFP human lung carcinoma growing on lung of nude mouse. Whole-body image.

Melanoma, human

LOXMIVI

SK-mel-5

Melanoma, mouse

B16-F10

B16F0

Human LOX-1-GFP melanoma metastasized to lung (A), lymp node (B), bone (C) and adrenal gland (D) of nude mouse.

Muscle, mouse

C2C12

Osteosarcoma

OST

L-SLM

Ovarian, human

OVCAR-8

RGMI-186

SKOV-3

Ovarian, hamster

CHOK-1

Pancreas, human

Bx-PC-3

MIA-PaCa-2

ASPC -1

Cfpaca

PaCa-1

PaCa28

BxPC-3-GFP pancreatic carcinoma growing on pancreas with liver and spleen metastasis. Whole-body image. White arrows - primary tumor, red arrow - spleen metastasis, yellow arrow - liver metastasis.

Prostate, human

PC-3

DU-145

LnCaP

MDA PCa 2b

Prostate, rat

Dunning-3327

	PC-3 prostate cancer

Week 2	Week 3	Week 4

White solid arrows: Primary tumor; white hollow arrows: mesenteric lymph nodes; pink arrows: inguinal lymph nodes, green arrow: axial lymph node

PC-3 growing in the tibia

Renal, human

SN12C

A498

Stomach, human

NUGC-4

NUGC-4-GFP human stomach tumor in nude mouse. Primary tumor- red arrow, lymph node metastases - white arrows

Tounge, human

SCC-25

IN VIVO RFP TUMOR MODELS

GFP Publications

1. Chishima, T., Miyagi, Y., Wang, X., Yamaoka, H., Shimada, H., Moossa, A.R., and Hoffman, R.M. Cancer invasion and micrometastasis visualized in live tissue by green fluorescent protein expression. Cancer Res. 57, 2042-2047, 1997.

2. Chishima, T., Miyagi, Y., Wang, X., Baranov, E., Tan, Y., Shimada, H., Moossa, A.R., and Hoffman, R.M. Metastatic patterns of lung cancer visualized live and in process by green fluorescent protein expression. Clinical & Experimental Metastasis 15, 547-552, 1997.

3. Chishima, T., Miyagi, Y., Wang, X., Tan, Y., Shimada, H., Moossa, A.R., and Hoffman, R.M. Visualization of the metastatic process by green fluorescent protein expression. Anticancer Res. 17, 2377-2384, 1997.

4. Chishima, T., Yang, M., Miyagi, Y., Li, L., Tan, Y., Baranov, E., Shimada, H., Moossa, A.R., Penman, S., and Hoffman, R.M. Governing step of metastasis visualized in vitro. Proc. Natl. Acad. Sci. USA 94, 11573-11576, 1997.

5. Chishima, T., Miyagi, Y., Li, L., Tan, Y., Baranov, E., Yang, M., Shimada, H., Moossa, A.R., and Hoffman, R.M. The use of histoculture and green fluorescent protein to visualize tumor cell host interaction. In Vitro Cell. Dev. Biol. 33, 745-747, 1997.

6. Yang, M., Hasegawa, S., Jiang, P., Wang, X., Tan, Y., Chishima, T., Shimada, H., Moossa, A.R., and Hoffman, R.M. Widespread skeletal metastatic potential of human lung cancer revealed by green fluorescent protein expression. Cancer Res. 58, 4217-4221, 1998.

7. Yang, M., Jiang, P., Sun, F.X., Hasegawa, S., Baranov, E., Chishima, T., Shimada, H., Moossa, A.R., and Hoffman, R.M. A fluorescent orthotopic bone metastasis model of human prostate cancer. Cancer Res. 59, 781-786, 1999.

8. Hoffman, R.M. Orthotopic transplant mouse models with green fluorescent protein-expressing cancer cells to visualize metastasis and angiogenesis. Cancer and Metastasis Reviews 17, 271-277, 1999.

9. Hoffman, R.M. Green fluorescent protein to visualize cancer progression and metastasis. Methods in Enzymology, Green Fluorescent Protein, Vol. 302. Ed: P. Michael Conn, Academic Press, San Diego, pp. 20-31, 1999.

10. Naumov, G.N., Wilson, S.M., MacDonald, I.C., Schmidt, E.E., Morris, V.L., Groom, A.C., Hoffman, R.M., and Chambers, A.F. Cellular expression of green fluorescent protein, coupled with high-resolution in vivo videomicroscopy, to monitor steps in tumor metastasis. Journal of Cell Science 112, 1835-1842, 1999.

11. Yang, M., Chishima, T., Baranov, E., Shimada, H., Moossa, A.R., and Hoffman, R.M. Green fluorescent protein: A new light to visualize metastasis and angiogenesis in cancer. Proc. of SPIE Conference on Molecular Imaging: Reporters, Dyes, Markers and Instrumentation 3500, 117-124, 1999.

12. Yang, M., Jiang, P., An, Z., Baranov, E., Li, L., Hasegawa, S., Al-Tuwaijri, M., Chishima, T., Shimada, H., Moossa, A.R., Hoffman, R.M. Genetically fluorescent melanoma bone and organ metastasis models. Clinical Cancer Res. 5, 3549-3559, 1999.

13. Yang, M., Chishima, T., Wang, X., Baranov, E., Shimada, H., Moossa, A.R., and Hoffman, R.M. Multi-organ metastatic capability of Chinese hamster ovary cells revealed by green fluorescent protein (GFP) expression. Clinical & Experimental Metastasis, 17, 417-422, 1999.

14. Hoffman, R.M. Visualization of metastasis in orthotopic mouse models with green fluorescent protein. In: Relevance of Tumor Models in Anticancer Drugs Development. Eds.: Fiebig, H.H., Burger, A.M., Kluwver Academic Publishers, Dordrecht, The Netherlands, Vol. 54, 81-87, 1999.

15. Yang, M., Baranov, E., Jiang, P., Sun, F-X., Li, X-M., Li, L., Hasegawa, S., Bouvet, M., Al-Tuwaijri, M., Chishima, T., Shimada, H., Moossa, A.R., Penman, S., Hoffman, R.M. Whole-body optical imaging of green fluorescent protein-expressing tumors and metastases. Proc. Natl. Acad. Sci. USA 97, 1206-1211, 2000.

17. Hoffman, R.M. Correspondence re: Initial stages of tumor cell-induced angiogenesis: Evaluation via skin window chambers in rodent models. Journal of the National Cancer Institute 92, 1445, 2000.

18. Yang, M., Baranov, E., Shimada, H., Moossa, A.R., and Hoffman, R.M. External optical imaging freely-moving mice with green fluorescent protein-expressing metastatic tumors. Proc. of SPIE Proceedings 3921, 256-259, 2000.

20. Yang, M., Baranov, E., Moossa, A.R., Penman, S., Hoffman, R.M. Visualizing gene expression by whole-body fluorescence imaging. Proc. Natl. Acad. Sci. USA 97, 12278-12282, 2000.

21. Hasegawa, S., Yang, M., Chishima, T., Shimada, H., Moossa, A.R., Hoffman, R.M. In vivo tumor delivery of the green fluorescent protein gene to report future occurrence of metastasis. Cancer Gene Therapy 7, 1336-1340, 2000.

22. Rashidi, B., Yang, M., Jiang, P., Baranov, E., An, Z., Wang, X., Moossa, A.R., and Hoffman, R.M. A highly metastatic Lewis lung carcinoma orthotopic green fluorescent protein model. Clinical & Experimental Metastasis 18, 57-60, 2000.

23. Bouvet, M., Yang, M., Nardin, S., Wang, X., Jiang, P., Baranov, E., Moossa, A.R., Hoffman, R.M. Chronologically-specific metastatic targeting of human pancreatic tumors in orthotopic models. Clinical & Experimental Metastasis 18, 213-218, 2000.

24. Yang, M., Baranov, E., Li, X-M., Wang, J-W., Jiang, P., Li, L., Moossa, A.R., Penman, S., Hoffman, R.M. Whole‑body and intravital optical imaging of angiogenesis in orthotopically implanted tumors. Proc. Natl. Acad. Sci. USA 98, 2616-2621, 2001.

25. Robinson, K. Imaging system captures whole-body GFP images. Biophotonics International, April, 54-55, 2001.

26. Pfeifer, A., Kessler, T., Yang, M., Baranov, E., Kootstra, N., Cheresh, D.A., Hoffman, R.M., and Verma, I.M. Transduction of liver cells by lentiviral vectors: Analysis in living animals. Molecular Therapy 3, 319-322, 2001.

27. Hoffman, Robert M. Visualization of GFP-expressing tumors and metastasis in vivo. BioTechniques 30, 1016-1016, 2001.

28. Hutchinson, E. Fluorescence imaging of tumour metastasis and angiogenesis. The Lancet Oncology, 2, 254, 2001.

29. McCann, J. Jellyfish protein gives new glow to tumor imaging. Journal of the National Cancer Institute 93, 976-977, 2001.

30. Lee, N.C., Bouvet, M., Nardin, S., Jiang, P., Baranov, E., Rashidi, B., Yang, M., Wang, X., Moossa, A.R., and Hoffman, R.M. Antimetastatic efficacy of adjuvant gemcitabine in a pancreatic cancer orthotopic model. Clinical & Experimental Metastasis 18, 379-384, 2001.

31. Kamiyama, M., Ichikawa, Y., Ishikawa, T., Chishima, T., Hasegawa, S., Hamaguchi, Y., Nagashima, Y., Miyagi, Y., Mitsuhashi, M., Hyndman, D., Hoffman, R.M., Ohki, S., and Shimada, H. VEGF-receptor antisense therapy inhibits angiogenesis and metastasis peritoneal dissemination of human gastric cancer in nude mice. Cancer Gene Therapy, 9, 197-201, 2002.

32. Li, X-M., Wang, J-W., An, Z., Yang, M. Baranov, E., Jiang, P., Sun, F-X., Moossa, A.R., and Hoffman, R.M. Optically-imageable metastatic model of human breast cancer. Clinical & Experimental Metastasis, 19, 347-350, 2002.

33. Bouvet, M., Wang, J-W., Nardin, S.R., Nassirpour, R., Yang, M., Baranov, E., Jiang, P., Moossa, A.R., and Hoffman, R.M. Real-time optical imaging of primary tumor growth and multiple metastatic events in a pancreatic cancer orthotopic model. Cancer Research 62, 1534-1540, 2002.

34. Yang, M., Baranov, E., Wang, J-W., Jiang, P., Wang, X., Sun, F-X., Bouvet, M., Moosa, A.R., Penman, S., and Hoffman, R.M. Direct external imaging of nascent cancer, tumor progression, angiogenesis, and metastasis on internal organs in the fluorescent orthotopic model. Proc. Natl. Acad. Sci. USA 99, 3824-3829, 2002

35. Schmitt, C.A., Fridman, J.S., Yang, M., Baranov, E., Hoffman, R.M., and Lowe, S.W. Dissecting p53 tumor suppressor functions in vivo. Cancer Cell, 1, 289-298, 2002

36. Hoffman, R.M., Green Fluorescent Protein Imaging of Tumor Cells in Mice. Lab Animal, 31, 4, 34-41, 2002.

37. Schmitt, C.A., Fridman, J.S., Yang, M., Lee, S., Baranov, E., Hoffman R.M., and Lowe, S.W. A Senescence Program Contolled by p53 and p16^INK4a Contribures to the Outcome of Cancer Therapy. Cell, 109, 335-346, May 3, 2002.

38. Hoffman, R.M. Watching real-time metastasis in vivo. Trends in Molecular Medicine, 8(7), 354-355, 2002.

39. Hoffman, R.M. Whole-body fluorescence imaging with Green Fluorescence Protein. In: Hicks, B., ed. Green Fluorescent Protein Applications and Protocols: Methods in Molecular Biology. Totowa, NJ: Humana Press, 135-148, 2002.

40. Hoffman, R. M., In vivo imaging of metastatic cancer with fluorescent proteins. Journal of Cell Death and Differentiation. 9, 786-789, 2002.

41. Zhou, J-H., Rosser, C.J., Tanaka, M., Yang, M., Baranov, E., Hoffman R,m., Benedict, W.F. Visualizing sperficial human bladder cancer cell growth in vivo by green fluorescent protein expression. Cancer Gene Therapy 9, 681-686, 2002.

42. Hoffman, R.M. Green fluorescent protein imaging of tumor growth, metastasis, and angiogenesis in mouse models. Lancet Oncology 3, 546-556, 2002.

IN VIVO RFP TUMOR MODELS

GFP Publications