OLYMPUS OV100 IMAGING SYSTEM: BROCHURE AND SPECIFICATION [PDF]

OLYMPUS OV100 IMAGING SYSTEM FEATURES [PDF]

Dynamic subcellular imaging of intravascular trafficking of dual-color cancer cells (labeled with GFP in the nucleus and RFP in the cytoplasm) in vessels in live mice.  Interaction of cancer cells with blood vessels depends on the ability of the cytoplasm and nucleus to deform, interaction of the cancer cells with endothelial cells, and the diameter of the vessel.  Real-time intravascular trafficking of HT1080-GFP-RFP cells was observed in the epigastric vein in a skin flap of live mice using the Olympus OV100 Small Animal Imaging System.  The cytoplasm and nuclei were slightly elongated in normal size capillaries.  In a larger vessel, the cells and nuclei remained spherical.  In the larger vessels, some cells attached to the vessel walls.  Some of the cells subsequently detached and began crawling on the vessel surface (Supplemental movies 1 and 2).  MMT-GFP-RFP cells extravasated by initially protruding a cytoplasmic projection with the nuclei exiting via the projection (Supplementary movie 3) (Yamauchi, K., et al. Development of real-time subcellular dynamic multicolor imaging of cancer cells in live mice with a variable-magnification whole-mouse imaging system.  Cancer Research 66, 4208-4214, 2006).

To see the movies click on the pictures: