Untitled Document

IN VIVO GFP TUMOR MODELS
WHOLE-BODY IMAGING OF ORTHOTOPIC PC-3-GFP IN NUDE MICE

Bladder, human	Breast cancer, human	Colon cancer, human
Cell type-HTB-9 UC-14 KU-7-GFP human bladder carcinoma growing on nude mouse bladder MX-1-GFP and –RFP breast carcinoma growing on mammary fat pads of SCID mouse. Whole-body image.	Cell types - MDA-MB-435,MDA-MB-231,MDA-MB-468,MCF-7,MX-1 Primary breast tumor MDA-MB-435 and lymph node metastases expressing GFP or RFP	Cell types - HCT-116,COLO-205,SW-620,Ls-180 ,HCT-15 ,KM-12, WiDr, HT-29,CaCo-2,RKO AC3488-GFP-tumor growing on colon and metastasized to liver and lymph nodes of nude mouse.Whole-body image.. Primary tumor - red arrow, liver metastases - white arrow, lymph node - yellow arrow.
Larynx, human	Lung cancer, human	Melanoma, human
Cell type- Hep-2 Primary tumor growth of Hep-2	Cell types - H-460,HOP-62,EKVX,Anip-973,A549 H-460-GFP human lung carcinoma growing on lung of nude mouse. Whole-body image.	Cell types - LOXMIVI,SK-mel-5 Human LOX-1-GFP melanoma metastasized to lung (A), lymp node (B), bone (C) and adrenal gland (D) of nude mouse.
Pancreas, human	Prostate, human	Stomach, human
Cell types - Bx-PC-3,MIA-PaCa-2, ASPC -1,Cfpaca,PaCa-1, PaCa28 BxPC-3-GFP pancreatic carcinoma growing on pancreas with liver and spleen metastasis. Whole-body image. White arrows - primary tumor, red arrow - spleen metastasis, yellow arrow - liver metastasis.	Cell types - PC-3,DU-145,LnCaP,MDA PCa 2b PC-3 prostate cancer. White solid arrows: Primary tumor; white hollow arrows: mesenteric lymph nodes; pink arrows: inguinal lymph nodes, green arrow: axial lymph node	Cell types- NUGC-4 NUGC-4-GFP human stomach tumor in nude mouse. Primary tumor- red arrow, lymph node metastases - white arrows
CNS, human	Fibrosarcoma, human	Hepatoma, human
Cell types - SNB-19,324,U87-MG	Cell types - HT-1080	Cell types - SMMC-7721
Melanoma, mouse	Muscle, mouse	Osteosarcoma
Cell types - B16-F10,B16F0	Cell types - C2C12	Cell types - OST,L-SLM
Ovarian, human	Ovarian, hamster	Renal, human
Cell types - OVCAR-8,RGMI-186, SKOV-3	Cell types - CHOK-1	Cell types - SN12C, A498
Tounge, human
Cell types - SCC-25

GFP Publications
1. Chishima, T., Miyagi, Y., Wang, X., Yamaoka, H., Shimada, H., Moossa, A.R., and Hoffman, R.M. Cancer invasion and micrometastasis visualized in live tissue by green fluorescent protein expression. Cancer Res. 57, 2042-2047, 1997.

2. Chishima, T., Miyagi, Y., Wang, X., Baranov, E., Tan, Y., Shimada, H., Moossa, A.R., and Hoffman, R.M. Metastatic patterns of lung cancer visualized live and in process by green fluorescent protein expression. Clinical & Experimental Metastasis 15, 547-552, 1997.

3. Chishima, T., Miyagi, Y., Wang, X., Tan, Y., Shimada, H., Moossa, A.R., and Hoffman, R.M. Visualization of the metastatic process by green fluorescent protein expression. Anticancer Res. 17, 2377-2384, 1997.

4. Chishima, T., Yang, M., Miyagi, Y., Li, L., Tan, Y., Baranov, E., Shimada, H., Moossa, A.R., Penman, S., and Hoffman, R.M. Governing step of metastasis visualized in vitro. Proc. Natl. Acad. Sci. USA 94, 11573-11576, 1997.

5. Chishima, T., Miyagi, Y., Li, L., Tan, Y., Baranov, E., Yang, M., Shimada, H., Moossa, A.R., and Hoffman, R.M. The use of histoculture and green fluorescent protein to visualize tumor cell host interaction. In Vitro Cell. Dev. Biol. 33, 745-747, 1997.

6. Yang, M., Hasegawa, S., Jiang, P., Wang, X., Tan, Y., Chishima, T., Shimada, H., Moossa, A.R., and Hoffman, R.M. Widespread skeletal metastatic potential of human lung cancer revealed by green fluorescent protein expression. Cancer Res. 58, 4217-4221, 1998.

7. Yang, M., Jiang, P., Sun, F.X., Hasegawa, S., Baranov, E., Chishima, T., Shimada, H., Moossa, A.R., and Hoffman, R.M. A fluorescent orthotopic bone metastasis model of human prostate cancer. Cancer Res. 59, 781-786, 1999.

8. Hoffman, R.M. Orthotopic transplant mouse models with green fluorescent protein-expressing cancer cells to visualize metastasis and angiogenesis. Cancer and Metastasis Reviews 17, 271-277, 1999.

9. Hoffman, R.M. Green fluorescent protein to visualize cancer progression and metastasis. Methods in Enzymology, Green Fluorescent Protein, Vol. 302. Ed: P. Michael Conn, Academic Press, San Diego, pp. 20-31, 1999.

10. Naumov, G.N., Wilson, S.M., MacDonald, I.C., Schmidt, E.E., Morris, V.L., Groom, A.C., Hoffman, R.M., and Chambers, A.F. Cellular expression of green fluorescent protein, coupled with high-resolution in vivo videomicroscopy, to monitor steps in tumor metastasis. Journal of Cell Science 112, 1835-1842, 1999.

11. Yang, M., Chishima, T., Baranov, E., Shimada, H., Moossa, A.R., and Hoffman, R.M. Green fluorescent protein: A new light to visualize metastasis and angiogenesis in cancer. Proc. of SPIE Conference on Molecular Imaging: Reporters, Dyes, Markers and Instrumentation 3500, 117-124, 1999.

12. Yang, M., Jiang, P., An, Z., Baranov, E., Li, L., Hasegawa, S., Al-Tuwaijri, M., Chishima, T., Shimada, H., Moossa, A.R., Hoffman, R.M. Genetically fluorescent melanoma bone and organ metastasis models. Clinical Cancer Res. 5, 3549-3559, 1999.

13. Yang, M., Chishima, T., Wang, X., Baranov, E., Shimada, H., Moossa, A.R., and Hoffman, R.M. Multi-organ metastatic capability of Chinese hamster ovary cells revealed by green fluorescent protein (GFP) expression. Clinical & Experimental Metastasis, 17, 417-422, 1999.

14. Hoffman, R.M. Visualization of metastasis in orthotopic mouse models with green fluorescent protein. In: Relevance of Tumor Models in Anticancer Drugs Development. Eds.: Fiebig, H.H., Burger, A.M., Kluwver Academic Publishers, Dordrecht, The Netherlands, Vol. 54, 81-87, 1999.

15. Yang, M., Baranov, E., Jiang, P., Sun, F-X., Li, X-M., Li, L., Hasegawa, S., Bouvet, M., Al-Tuwaijri, M., Chishima, T., Shimada, H., Moossa, A.R., Penman, S., Hoffman, R.M. Whole-body optical imaging of green fluorescent protein-expressing tumors and metastases. Proc. Natl. Acad. Sci. USA 97, 1206-1211, 2000.

16. Dove, A. Illuminating cancer models. Nature Biotechnology 18, 261, 2000.

17. Hoffman, R.M. Correspondence re: Initial stages of tumor cell-induced angiogenesis: Evaluation via skin window chambers in rodent models. Journal of the National Cancer Institute 92, 1445, 2000.

18. Yang, M., Baranov, E., Shimada, H., Moossa, A.R., and Hoffman, R.M. External optical imaging freely-moving mice with green fluorescent protein-expressing metastatic tumors. Proc. of SPIE Proceedings 3921, 256-259, 2000.

19. Corrigendum, BioFeedback. Biotechniques 29, 544, September 2000.

20. Yang, M., Baranov, E., Moossa, A.R., Penman, S., Hoffman, R.M. Visualizing gene expression by whole-body fluorescence imaging. Proc. Natl. Acad. Sci. USA 97, 12278-12282, 2000.

21. Hasegawa, S., Yang, M., Chishima, T., Shimada, H., Moossa, A.R., Hoffman, R.M. In vivo tumor delivery of the green fluorescent protein gene to report future occurrence of metastasis. Cancer Gene Therapy 7, 1336-1340, 2000.

22. Rashidi, B., Yang, M., Jiang, P., Baranov, E., An, Z., Wang, X., Moossa, A.R., and Hoffman, R.M. A highly metastatic Lewis lung carcinoma orthotopic green fluorescent protein model. Clinical & Experimental Metastasis 18, 57-60, 2000.

23. Bouvet, M., Yang, M., Nardin, S., Wang, X., Jiang, P., Baranov, E., Moossa, A.R., Hoffman, R.M. Chronologically-specific metastatic targeting of human pancreatic tumors in orthotopic models. Clinical & Experimental Metastasis 18, 213-218, 2000.

24. Yang, M., Baranov, E., Li, X-M., Wang, J-W., Jiang, P., Li, L., Moossa, A.R., Penman, S., Hoffman, R.M. Whole‑body and intravital optical imaging of angiogenesis in orthotopically implanted tumors. Proc. Natl. Acad. Sci. USA 98, 2616-2621, 2001.

25. Robinson, K. Imaging system captures whole-body GFP images. Biophotonics International, April, 54-55, 2001.

26. Pfeifer, A., Kessler, T., Yang, M., Baranov, E., Kootstra, N., Cheresh, D.A., Hoffman, R.M., and Verma, I.M. Transduction of liver cells by lentiviral vectors: Analysis in living animals. Molecular Therapy 3, 319-322, 2001.

27. Hoffman, Robert M. Visualization of GFP-expressing tumors and metastasis in vivo. BioTechniques 30, 1016-1016, 2001.

28. Hutchinson, E. Fluorescence imaging of tumour metastasis and angiogenesis. The Lancet Oncology, 2, 254, 2001.

29. McCann, J. Jellyfish protein gives new glow to tumor imaging. Journal of the National Cancer Institute 93, 976-977, 2001. [HTML]

30. Lee, N.C., Bouvet, M., Nardin, S., Jiang, P., Baranov, E., Rashidi, B., Yang, M., Wang, X., Moossa, A.R., and Hoffman, R.M. Antimetastatic efficacy of adjuvant gemcitabine in a pancreatic cancer orthotopic model. Clinical & Experimental Metastasis 18, 379-384, 2001.
31. Zhao, M., Yang.M., Baranov, E., Wang, X., Penman, S., Moossa, A.R., Hoffman, R.M. Spatial-temporal imaging of bacterial infection and antibiotic response in intact animals. Proc. Natl. Acad. Sci. USA 98, 9814-9818, 2001.
32.    Hoffman, R.M.   Green Fluorescent Protein for Metastasis Research. In: Brooks, S., and Schumacher, U., eds. Metastasis Research Protocols. Vol. II. Analysis of Cell Behavior In Vitro and In Vivo. Totowa, NJ: Humana Press Inc., 285-298, 2001.
33.    Hoffman, R.M. GFP-Expressing Metastatic-Cancer Mouse Models. In: Teicher, B., ed. Tumor Models in Cancer Research. Totowa, NJ: Humana Press Inc., 99-112, 2002.

34. Kamiyama, M., Ichikawa, Y., Ishikawa, T., Chishima, T., Hasegawa, S., Hamaguchi, Y., Nagashima, Y., Miyagi, Y., Mitsuhashi, M., Hyndman, D., Hoffman, R.M., Ohki, S., and Shimada, H. VEGF-receptor antisense therapy inhibits angiogenesis and metastasis peritoneal dissemination of human gastric cancer in nude mice. Cancer Gene Therapy, 9, 197-201, 2002.
35. Yang, M., Baranov, E., Wang, J-W., Jiang, P., Wang, X., Sun, F-X., Bouvet, M., Moossa, A.R., Penman, S., and Hoffman, R.M. Direct external imaging of nascent cancer, tumor progression, angiogenesis, and metastasis on internal organs in the fluorescent orthotopic model. Proc. Natl. Acad. Sci. USA 99, 3824-3829, 2002.
36. Bouvet, M., Wang, J-W., Nardin, S.R., Nassirpour, R., Yang, M., Baranov, E., Jiang, P., Moossa, A.R., and Hoffman, R.M. Real-time optical imaging of primary tumor growth and multiple metastatic events in a pancreatic cancer orthotopic model. Cancer Research 62, 1534-1540, 2002.
37. Hoffman, R.M. Green fluorescent protein imaging of tumor cells in mice. Lab Animal 31, 34-41, 2002.
38. Bouvet, M., Nardin, S.R., Burton, D.W., Lee, N.C., Yang, M., Wang, X., Baranov, E., Behling, C., Moossa, A.R., Hoffman, R.M., and Deftos L.J. Parathyroid hormone-related protein as a novel tumor marker in pancreatic adenocarcinoma. Pancreas 24, 284-290, 2002.
39. Schmitt, C.A., Fridman, J.S., Yang, M., Baranov, E., Hoffman, R.M. and Lowe, S.W. Dissecting p53 tumor suppressor functions in vivo. Cancer Cell 1, 289-298, 2002.
40. Schmitt, C.A., Yang, M., Fridman, J.S., Baranov, E., Hoffman, R.M., and Lowe, S.W. Senescence program controlled by p53 and p16^INK4a contributes to the outcome of cancer therapy. Cell 109, 335–346, 2002.
41. Li, X-M., Wang, J-W., An, Z., Yang, M. Baranov, E., Jiang, P., Sun, F-X., Moossa, A.R., and Hoffman, R.M. Optically imageable metastatic model of human breast cancer. Clinical & Experimental Metastasis, 19, 347-350, 2002.
42. Hoffman, R.M. Watching real-time metastasis in vivo. Trends in Molecular Medicine, 8(7), 354-355, 2002.
43. Hoffman, R.M. Whole-body fluorescence imaging with Green Fluorescence Protein. In: Hicks, B.W., ed. Methods in Molecular Biology, Vol. 183: Green Fluorescent Protein: Applications and Protocols. Totowa, NJ: Humana Press, 135-148, 2002.
44. Hoffman, R.M. In vivo imaging of metastatic cancer with fluorescent proteins. Journal of Cell Death and Differentiation 9, 786-789, 2002.
45. Zhou, J-H., Rosser, C.J., Tanaka, M., Yang, M., Baranov, E., Hoffman, R.M., Benedict, W.F. Visualizing superficial human bladder cancer cell growth in vivo by green fluorescent protein expression. Cancer Gene Therapy 9, 681-686, 2002.
46. Hoffman, R.M. Green fluorescent protein imaging of tumour growth, metastasis, and angiogenesis in mouse models. Lancet Oncology 3, 546-556, 2002.
47. Saito, N., Zhao, M., Li, L., Baranov, E., Yang, M., Ohta, Y., Katsuoka, K., Penman, S. and Hoffman, R. High efficiency genetic modification of hair follicles and growing hair shafts. Proc. Natl. Acad. Sci. 99, 13120-13124, 2002
48. Sun, F-X., Tohgo, A., Bouvet, M., Yagi, S., Nassirpour, R., Moossa, A.R., and Hoffman, R.M. Efficacy of camptothecin analog DX-8951f (Exatecan Mesylate) on human pancreatic cancer in an orthotopic metastatic model. Cancer Research 63, 80-85, 2003.
49. Wang, J-W., Yang, M., Wang, X., Sun, F-X., Li, X-M., Yagi, S., and Hoffman, R.M. Antimetastatic efficacy of oral 5-FU imaged by green fluorescent protein in real time. Anticancer Research 23, 1-6, 2003.
50. Yamamoto, N., Yang, M., Jiang, P., Tsuchiya, H., Tomita, K., Moossa, A.R., and Hoffman, R.M. Real-time GFP imaging of spontaneous HT-1080 fibrosarcoma lung metastases. Clin. Exp. Metastasis 20, 181-185, 2003.
51. John, C.M., Leffler, H., Kahl-Knutsson, B., Svensson, I., Jarvis, G.A. Truncated Galectin-3 Inhibits Tumor Growth and Metastasis in Orthotopic Nude Mouse Model of Human Breast Cancer. Clinical Cancer Res. 9, 3808-3814, 2003
52. Glinskii, A.B., Smith, B.A., Jiang, P., Li, X-M., Yang, M., Hoffman, R.M., Glinsky, G.V. Viable circulating metastatic cells produced in orthotopic but not ectopic prostate cancer models. Cancer Res. 63, 4239-4243, 2003.
53. Katz, M.H., Spivack, D.E., Takimoto, S., Fang, B., Burton, D.W., Moosa, A.R., Hoffman, R.M., and Bouvet, M. Gene therapy of pancreatic cancer with green fluorescent protein and tumor necrosis factor-related apoptosis-inducing ligand fusion gene expression driven by a human telomerase reverse transcriptase promoter. Annals of Surgical Oncology 10, 762-772, 2003.
54. Katz, M., Takimoto, S., Spivack, D., Moossa, A.R., Hoffman, R.M., Bouvet, M. A novel red fluorescent protein orthotopic pancreatic cancer model for the preclinical evaluation of chemotherapeutics. J.Surg. Res. 113, 151-160, 2003.
55. Katz, M.H., Bouvet, M., Takimoto, S., Spivac, D., Moossa, A.R., Hoffman, R.M. Selective antimetastatic activity of cytosine analog CS-682 in a red fluorescent protein orthotopic model of pancreatic cancer. Cancer Res. 63, 5521-5525, 2003.
56. Goodison, S., Kawai, K., Hihara, J., Jiang, P., Yang, M., Urquidi, V., Hoffman, R.M., and Tarin, D. Prolonged dormancy and site-specific growth potential of cancer cells spontaneously disseminated from non-metastatic breast tumors revealed by labeling with green fluorescent protein. Clinical Cancer Res. 9, 3808-3814, 2003.
57. Yamamoto, N., Yang, M., Jiang, P., Xu, M., Tsuchiya, H., Tomita, K., Moossa, A.R., and Hoffman, R.M. Real-time imaging of individual color-coded metastatic colonies in vivo. Clin. Exp. Metastasis 20(7), 633-638, 2003.
58. Yamamoto, N., Yang, M., Jiang, P., Xu, M., Tsuchiya, H., Tomita, K., Moossa, A.R., and Hoffman, R.M. Determination of clonality of metastasis by cell-specific color-coded fluorescent-protein imaging. Cancer Research 63, 7785-7790, 2003.
59. Yang, M., Li, L., Jiang, P., Moossa, A.R., Penman, S., and Hoffman, R.M. Dual-color fluorescence imaging distinguishes tumor cells from induced host angiogenic vessels and stromal cells. Proc. Natl. Acad. Sci. USA 100, 14259-14262, 2003.
60. Hoffman, R.M., and Yagi, S. In vivo single-cell imaging. Bio-venture (Japan) 3, 77-78, 2003
61. Mitsiades, C.S., Mitsiades, N.S., Bronson, R.T., Chauhan, D., Munshi, N., Treon, S.P., Maxwell, C.A., Pilarski, L., Hideshima, T., Hoffman, R.M., and Anderson, K.C. Fluorescence imaging of multiple myeloma cells in a clinically relevant SCID/NOD in vivo model: biologic and clinical implications. Cancer Research 63, 6689-6696, 2003.
62. Katz, M., Spivac, D., Takimoto, S., Bouvet, M., Moosa, A.R., R.M. Hoffman. An imageable highly metastatic orthotopic red fluorescent protein model of pancreatic cancer. Clin. Exp. Metastasis 21, 7-12, 2004.
63. Katz, M.H., Bouvet, M., Takimoto, S., Spivack, D., Moossa, A.R., and Hoffman, R.M. Survival efficacy of adjuvant cytosine-analogue CS-682 in a fluorescent orthotopic model of human pancreatic cancer. Cancer Research 64, 1828-1833, 2004.
64. Hoffman, R. Multicolor in vivo imaging in mouse models of cancer. Preclinica. 2, 109-113, 2004.
65. Robinson, K. Dual-color fluorescence imaging distinguishes tumor from host. Biophotonics Intl., January, 56-57, 2004
66. Mihich, E. and Kaelin, W. Fifteenth Annual Pezcoller Symposium: Molecular in vivo visualization of cancer cells. Cancer research 64, 2929-2933, 2004
67. Hoffman, R.M. In vivo imaging with fluorescent proteins: THe new cell biology. Acta Histochemica 106, 77-87, 2004.
68. Yamamoto, N., Jiang, P., Yang, M., Xu, M., Yamauchi, K., Tsuchiya, H., Tomita, K., Wahl, G.M., Moossa, A.R., and Hoffman, R.M. Cellular dynamics visualized in live cells in vitro and in vivo by differential dual-color nuclear-cytoplasmic fluorescent-protein expression. Cancer Research 64, 4251-4256, 2004.
69. Yang, M., Amoh, Y., Li, L., Baranov, E., Wang, J-W., Jiang, P., Moossa, A.R., and Hoffman, R.M. Dual-color fluorescence imaging of tumor-host interaction with green and fluorescent proteins. In: Genetically Engineered and Optical Probes for Biomedical Applications II (Savitsky, A.P., Brovko, L.Y., Bornhop, D.J., Raghavachari, R., Achilefu, S.I., eds). Proceedings of SPIE Conference, 5329, 54-60, 2004.
70. Laakkonen, P., Akerman, M.E., Biliran, H., Yang, M., Ferrer, F., Karpanen, T., Hoffman, R.M., Ruoslani, E. Antitumor activity of a homing peptide that targets tumor lymphatics and tumor cells. Proc. Natl. Acad. Sci. USA 101, 9381-9386, 2004.
71. Wang, J-W., Yang, M., Yagi, S., and Hoffman, R.M. Oral 5-FU is a more effective antimetastatic agent than UFT. Anticancer Research 24, 1353-1360, 2004.
72. Amoh, Y., Li, L., Yang, M., Moosa, A.R., Katsuoka, K., Penman, S., and Hoffman, R.M., Nascent blood vessels in the skin arise from nestin-expressing hair follicle cells. Proc. Natl. Acad. Sci. USA 101, 13291-13295, 2004.
73. Bobek, V. Plachy, J., Pinterova, D., Kolotsova, K., Boubelik, M., Jiang, P. P., Yang, M., and Hoffman R. M. Development of a green fluorescent protein metastatic-cancer chick-embryo drug-screen model. Clinical & Experimental Metastasis 21, 347-352, 2004.
74. Yang, M., Reynoso, J., Jiang, P., Li, L., Moossa, A.R., and Hoffman, R.M. Transgenic nude mouse with ubiquitous green fluorescent protein _expression as a host for human tumors. Cancer Research 64, 8651-8656, 2004.
75. Hoffman, R.M. Imaging tumor angiogenesis with fluorescent proteins. Acta Pathologica, Microbiologica et Immunologica Scandinavica 112, 441-449, 2004.
76. Wang, J., Yang, M., and Hoffman, R.M. Visualizing portal vein metastatic trafficking to the liver with green fluorescent protein-expressing tumor cells. Anticancer Research 24, 3699-3702,2004.
77. Yamamoto, N., Yang, M., Jiang, P., Xu, M., Yamauchi, K., Tsuchiya, H., Tomita, K., Moossa, A.R., and Hoffman, R.M. Color coding cancer cells with fluorescent proteins to visualize in vivo cellular interaction in metastatic colonies. Anticancer Research 24, 4067-4072, 2004.
78. Bobek, V., Kolostova, K., Pinterov, D., Boubelik, M., Jiang, P., Yang, M., and Hoffman, R.M. Syngeneic lymph-node-targeting model of green fluorescent protein-expressing Lewis lung carcinoma. Clinical & Experimental Metastasis 21, 705-708, 2004.
79. Zhao, M., Yang, M., Li, X-M., Jiang, P., Li, S., Xu, M., and Hoffman, R.M. Tumor-targeting bacterial therapy with amino acid auxotrophs of GFP-expressing Salmonella typhimurium. Proc. of Natl. Acad. Sci USA 102, 755-760, 2005.
80. Burton, D.W., Geller, J., Yang, M., Jiang, P., Barken, I., Hastings, R.H., Hoffman, R.M., and Deftos, L.J. Monitoring of skeletal progression of prostate cancer by GFP imaging, X-ray, and serum OPG and PTHrP. The Prostate 62, 275-281, 2005. DOI: 10.1002/pros.20146.
81. Yang, M., Jiang, P., Yamamoto, N., Li, L., Geller, J., Moossa, A.R., and Hoffman, R.M. Real-time whole-body imaging of an orthotopic metastatic prostate cancer model expressing rad fluorescent protein. The Prostate 62, 374-379,2005. DOI #10.1002/pros.20125.
82. Deftos, L.J., Barken, I., Burton, D.W., Hoffman, R.M., and Geller, J. Direct evidence that PTHrP expression promotes prostate cancer progression in bone. Biochem. Biophys. Res. Commun. 327, 468-472, 2005.
83. Amoh, Y., Li, L., Yang, M., Jiang, P., Moossa, A.R., Katsuoka, K., Hoffman, R.M. Hair-follicle-derived blood vessels vascularize tumors in skin and are inhibited by doxorubicin. Cancer Research 65, 2337-2343, 2005.
84. Berezovskaya, O., Schimmer, A.D., Glinskii, A.B., Pinilla, C., Hoffman, R.M., Reed, J.C., and Glinky, G.V. Increased _expression of apoptosis inhibitor protein XIAP contributes to resistance to anoikis of human prostate cancer metastasis precursor cells. Cancer Research 65, 2378-2386, 2005.
85. Amoh, Y., Li, L., Katsuoka, K., Penman, S., and Hoffman, R.M. Multipotent nestin-positive, keratin-negative hair-follicle-bulge stem cells can form neurons. Proc. Natl. Acad. Sci. USA 102, 5530-5534, 2005.
86. Yamauchi, K., Yang, M., Jiang, P., Yamamoto, N., Xu, M., Amoh, Y., Tsuji, K., Bouvet, M., Tsuchiya, H., Tomita, K., Moossa, A.R., and Hoffman, R.M. Real-time in vivo dual-color imaging of intracapillary cancer cell and nucleus deformation and migration. Cancer Res. 65, 4246-4252, 2005.
87. Hoffman, R.M. Imaging of angiogenesis in vivo with fluorescent proteins: fluorescence imaging of angiogenesis. In: Meadows, Gary G., Ed. Integration/Interaction of Oncologic Growth. Book Series: Kaiser, H., Ed. Cancer Growth and Progression, 2^nd Ed., Vol. 15, pp. 37-45. Dordrecht, The Netherlands: Springer, 2005. ISBN #1-4020-3413-X.
88. Amoh, Y., Yang, M., Li, L., Reynoso, J., Bouvet, M., Moossa, A.R., Katsuoka, K., and Hoffman, R.M. Nestin-linked green fluorescent protein transgenic nude mouse for imaging human tumor angiogenesis. Cancer Res. 65, 5352-5357, 2005.
89. Hoffman, R.M. and Yang, M. Dual-color whole-body imaging in mice. Nature Biotechnology 23, 790-791, 2005.
90. Yang, M., Luiken, G., Baranov, E., and Hoffman, R.M. Facile whole-body imaging of internal fluorescent tumors in mice with an LED flashlight. Bio Techniques 39, 170-172, 2005.
91. Hoffman, R.M. Advantages of multi-color fluorescent proteins for whole-body and in in vivo cellular imaging. Journal of Biomedical Optics 10(4), 41202, AN #041202, 2005.
92. Hoffman, R.M. The multiple uses of fluorescent proteins to visualize cancer in vivo. Nature Reviews Cancer 5, 796-806, 2005.
93. Bouvet, M., Spernyak, J., Katz, M.H., Mazurchuk, R.V., Takimoto, S., Bernacki, R., Rustum, Y.M., Moossa, A.R., and Hoffman, R.M. High correlation of whole- body red fluorescent protein imaging and magnetic resonance imaging on an orthotopic model of pancreatic cancer. Cancer Research 65, 9829-9833, 2005.
94. Amoh, Y., Li, L., Campillo, R., Kawahara, K., Katsuoka, K., Penman, S., and Hoffman, R.M. Implanted hair follicle stem cells form Schwann cells that support repair of severed peripheral nerves. Proc. Natl. Acad. Sci. USA 102, 17734-17738,2005.
95. Hoffman, R.M. In vivo cell biology of cancer cells visualized with fluorescent proteins. In: “In vivo cellular and molecular imaging” for Current Topics in Developmental Biology, Vol. 70, pp. 121-144. Ahrens, Eric T., ed. London: Elsevier Life Sciences, 2005. DOI: 10.1016/S0070-2153(05)70006-5.
96. Hemman, M.T., Bric, A., Teruya-Feldstein, J., Herbst, A., Nilsson, J.A., Cordon-Cardo, C., Cleveland, J.L., Tansey, W.P., Lowe, S.W. Evasion of the p53 tumour surveillance network by tumour-derived MYC mutants. Nature 436, 807-811, 2005.
97. Tsuji, K., Yamauchi, K., Yang, M., Jiang, P., Bouvet, M., Endo, H., Kanai, Y., Yamashita, K., Moossa, A.R., and Hoffman, R.M. Dual-color imaging of nuclear-cytoplasmic dynamics, viability, and proliferation of cancer cells in the portal vein area. Cancer Res. 66, 303-306, 2006.
98. Pilch, J., Brown, D.M., Komatsu, M., Järvinen, T., Yang, M., Peters, D., Hoffman, R.M., and Ruoslahti, E. Peptides selected for binding to clotted plasma accumulate in tumor stroma and wounds. Proc. Natl. Acad. Sci. USA 103, 2800-2804, 2006.
99. Glinsky, G.V., Glinskii, A.B., Berezovskaya, O., Smith, B.A., Jiang, P., Li, X-M., Yang, M., Hoffman, R.M. Dual-color-coded imaging of viable circulating prostate carcinoma cells reveals genetic exchange between tumor cells in vivo, contributing to highly metastatic phenotypes. Cell Cycle 5, 191-197, 2006.
100. Tsuji, K., Yang, M., Jiang, P., Maitra, A., Kaushal, S., Yamauchi, K., Katz, M.D., Moossa, A.R.,    Hoffman, R.M, Bouvet, M. Common bile duct injection as a novel method for establishing RFP-expressing human pancreatic cancer in nude mice. Journal of the Pancreas 7, 193-199, 2006.
101. Yamauchi, K., Yang, M., Jiang, P., Xu, M., Yamamoto, N., Tsuchiya, H., Tomita, K., Moosa, A.R., Bouvet, M., and Hoffman, R.M. Development of real-time subcellular dynamic multicolor imaging of cancer cell trafficking in live mice with a variable-magnification whole-mouse imaging system. Cancer Res. 66, 4208-4214, 2006.
102. Yang, M., Burton, D.W., Geller, J., Hillegonds, D., Hastings, R.H., Deftos, L.J., and Hoffman, R.M. The bisphosphonate olpadronate inhibits skeletal prostate cancer progression in a green fluorescent protein nude mouse model. Clinical Cancer Research 12, 2602-2606, 2006.
103. Amoh, Y., Li, L., Tsuji, K., Moossa, A.R., Katsuoka, K., Hoffman, R.M., Bouvet, M. Dual-color imaging of nascent blood vessels vascularizing pancreatic cancer in an orthotopic model demonstrates antiangiogenesis efficacy of gemcitabine. J. Surgical Research 132, 164-169, 2006.
104. Jiang, P., Yamauchi, K., Yang., Tsuji, K., Xu, M., Maitra, A., Bouvet, M., and Hoffman, R.M. Tumor cells genetically labeled with GFP in the nucleus and RFP in the cytoplasm for imaging cellular dynamics. Cell Cycle 5, 1198-1201, 2006.
105. Seitz, G., Warmann, S.W., Fuchs, J., Mau-Holzmann U.A., Ruck, P., Heitmann, H., Hoffman, R.M., Mahrt, J., Muller, G.A., and Wessels, J.T. Visualization of xenotransplanted human rhabdomyosarcoma after transfection with red fluorescent protein. Journal of Pediatric Surgery 41, 1369-1376, 2006.
106. Hoffman, R.M., and Yang, M. Subcellular imaging in the live mouse. Nature Protocols 1, 775-782, 2006.
107. Hoffman, R.M., and Yang, M. Color-coded fluorescence imaging of tumor-host interactions. Nature Protocols 1, 928-935, 2006.
108. Hoffman, R.M., and Yang, M. Whole-body imaging with fluorescent proteins. Nature Protocols 1, 1429-1438, 2006.
109. Zhao, M., Yang, M., Ma, H., Li, X., Tan, X., Li, S., Yang, Z., and Hoffman, R.M. Targeted therapy with a Salmonella typhimurium leucine-arginine auxotroph cures orthotopic human breast tumors in nude mice. Cancer Research 66, 7647-7652, 2006.
110. Berezovska, O.P., Glinskii, A.B., Yang, Z., Li, X.M., Hoffman, R.M., and Glinsky, G.V. Essential role for activation of the Polycomb Group (PcG) protein chromatin silencing pathway in metastatic prostate cancer. Cell Cycle 5, 1886-1901, 2006.
111. Hoffman, R.M. Real-time subcellular imaging in live animals: New visible targets for cancer drug discovery. IDrugs 9, 632-635, 2006.
112. Amoh, Y., Nagakura, C., Maitra, A., Moosa, A.R., Katsuoka, K., Hoffman, R.M. and Bouvet, M. Dual-color imaging of nascent angiogenesis and its inhibition in liver metastases of pancreatic cancer. Anticancer Research 26, 3237-3242, 2006.
113. Bouvet, M., Tsuji, K., Yang, M., Jiang, P., Moossa, A.R., and Hoffman, R.M. In vivo color-coded imaging of the interaction of colon cancer cells and splenocytes in the formation of liver metastases. Cancer Research 66, 11293-11297, 2006
114. Hoffman, R.M. Multi-color fluorescence imaging of cellular dynamics in vivo. Proc. of SPIE 6098, 60980D1-60980D7, 2006.
115. Amoh, Y., Bouvet, M., Li, L., Tsuji, K., Moossa, A.R., Katsuoka, K., and Hoffman, R.M. Visualization of nascent tumor angiogenesis in lung and liver metastasis by differential dual-color fluorescence imaging in nestin-linked-GFP mice. Clin. Exp. Metastasis 23(7-8), 315-322, 2006.
116. Simberg, D., Duza, T., Park, J.H., Essler, M., Pilch, J., Zhang, L., Derfus, A.M., Yang, M., Hoffman, R.M., Bhatia, S., Sailor, M.J., and Ruoslahti, E. Biomimetic amplification of nanoparticle homing to tumors. Proc. Natl. Acad. Sci. USA 104, 932-936, 2007.
117. Hoffman, R.M., and Zhao, M. Whole-body imaging of bacterial infection and antibiotic response. Nature Protocols 1(6), 2988-2994, 2007.
118. Amoh, Y., Li, L., Moossa, A.R., Katsuoka, K., and Hoffman, R.M. Chemotherapy targets the hair-follicle vascular network but not the stem cells. J. Invest. Dermatol. 127, 11-15, 2007
119. Seitz, G., Warmann, S.W., Heitmann, H., Ruck, P., Hoffman, R.M., Jörg Fuchs, Wessels, J.T. Photodynamic therapy of rhabdomyosarcoma. Int. J. Oncol. 30, 615-620, 2007.
120. Yang, M., Jiang, P., Hoffman, R.M. Whole-body subcellular multicolor imaging. Proc. of SPIE 6449, 64490V1-64490V9, 2007
121. Bouvet, M., Hoffman R.M. Preclinical fluorescent mouse models of pancreatic cancer. Proc. of SPIE 6449, 64490X1-64490X11, 2007
122. Amoh, Y., Li, L., Katsuoka, K., Bouvet, M., and Hoffman, R.M. GFP-expressing vascularization of Gelfoam as a rapid in vivo assay of angiogenesis stimulators and inhibitors. BioTechniques 42, 294-298, 2007.
123. Hoffman, R.M. Noninvasive imaging for evaluation of the systemic delivery of capsid-modified adenovirus in an orthotopic model of advanced lung cancer. Cancer 109, 1213-1214, 2007.
124. Ji, Y., Hayashi, K., Amoh, Y., Tsuji, K., Yamauchi, K., Yamamoto, N., Tsuchiya, H., Tomita, K., Bouvet, M., and Hoffman, R.M. The camptothecin derivative CPT-11 inhibits angiogenesis in a dual-color imageable orthotopic metastatic nude mouse model of human colon cancer. Anticancer Res. 27, 713-718, 2007.
125. Yang, M., Jiang, P., and Hoffman, R.M. Whole-body subcellular multicolor imaging of tumor-host interaction and drug response in real time. Cancer Res. 67 , 5195-5200, 2007.
126. Hayashi, K., Yamauchi, K., Yamamoto, N., Tsuchiya, H., Tomita, K., Amoh, Y., Hoffman, R.M., and Bouvet, M. Dual-color imaging of angiogenesis and its inhibition in bone and soft tissue sarcoma. J. Surg. Res. 140, 165-170, 2007.
127. Zhao, M., Geller, J., Ma, H., Yang, M., Penman, S., Hoffman, R.M. Monotherapy with a tumor-targeting mutant of Salmonella typhimurium cures orthotopic metastatic mouse models of human prostate cancer. Proc. Natl. Acad. Sci. USA 104, 10170-10174, 2007.
128. Guo, X-N., Rajput, A., Rose, R., Hauser, J., Beko, A., Kuropatwinski, K., LeVea, C., Hoffman, R.M., Brattain, M.G., and Wang, J. Mutant PIK3CA-bearing colon cancer cells display increased metastasis in an orthotopic model. Cancer Res. 67, 5851-5858, 2007.
129. Liu, T., Ding, Y., Xie, W., Li, Z., Bai, X., Li, X., Fang, W., Ren, C., Wang, S., Hoffman, R.M., and Yao, K. An imageable metastatic treatment model of nasopharyngeal carcinoma. Clin. Cancer Res. 13, 3960-3967, 2007.
130. Ammons, W.S., Wang, J.W., Yang, Z., Tidmarsh, G.F., and Hoffman, R.M. A novel alkylating agent, glufosfamide, enhances the activity of gemcitabine in vitro and in vivo. Neoplasia 9, 625-633, 2007.
131. Hayashi, K., Jiang, P., Yamauchi, K., Yamamoto, N., Tsuchiya, H., Tomita, K., Moossa, A.R., Bouvet, M., and Hoffman, R.M. Real-time imaging of tumor-cell shedding and trafficking in lymphatic channels. Cancer Res. 67, 8223-8228, 2007.
132. Hoffman, R. M. Subcellular imaging of cancer calls in live mice. In: Reporter Genes: A Practical Guide, Voll. 411, pp. 121-130, Methods in Molecular Biology series, Anson, D.S., editor. Humana Press, 2007.
133. Yamauchi, K., Yang, M., Hayashi, K., Jiang, P., Yamamoto, N., Tsuchiya, H., Tomita, K., Moossa, A.R., Bouvet, M., and Hoffman, R.M. Imaging of nucleolar dynamics during the cell cycle of cancer cells in live mice. Cell Cycle 6, 2706-2708, 2007.
134. Hoffman, R.M. A better fluorescent protein for whole-body imaging. Trends in Biotechnology 26, 1-3, 2008.
135. Yamauchi, K., Yang, M., Hayashi, K., Jiang, P., Yamamoto, N., Tsuchiya, H., Tomita, K., Moossa, A.R., Bouvet, M., and Hoffman, R.M. Induction of cancer metastasis by cyclophosphamide pretreatment of host mice: an opposite effect of chemotherapy. Cancer Res. 68, 516-520, 2008.
136. Hoffman, R.M. Imaging in mice with fluorescent proteins: from macro to subcellular. Sensors 8, 1157-1173, 2008.
137. Seitz, G., Warmann, S.W., Fuchs, J., Heitmann, H., Mahrt, J., Busse, A-C., Ruck, P., Hoffman, R.M., and Wessels, J.T. Imaging of cell trafficking and metastasis of paediatric rhabdomyosarcoma. Cell Proliferation 41, 365-374, 2008.
138. Rajput, A., Dominguez San Martin, I., Rose, R., Beko, A., Levea, C., Sharratt, E., Mazurchuk, R., Hoffman, R.M., Brattain, M.G., and Wang, J. Characterization of HCT116 human colon cancer cells in an orthotopic model. J. Surg. Res. 147, 276-281, 2008.